Xess, ImmaculataMathur, PurvaSirka, Chandra ShekarBanerjee, Uma2009-05-272009-05-272004-06-05Xess I, Mathur P, Sirka CS, Banerjee U. Comparison of trypsin treatment method and standard laboratory technique for diagnosis of dermatomycosis. The Southeast Asian Journal of Tropical Medicine and Public Health. 2004 Jun; 35(2): 396-8http://imsear.searo.who.int/handle/123456789/35776The Southeast Asian Journal of Tropical Medicine and Public Health.Dermatomycosis is prevalent worldwide. Discrepancy between microscopic examination and culture findings can create problems in the diagnosis of this common infection. In this study, samples from 60 patients were processed after trypsin treatment and examined by neutral red staining to distinguish viable and non-viable fungal elements. The trypsin treatment method was compared with standard laboratory techniques. A higher number of direct-microscopy-positive, culture-negative samples were obtained without trypsin treatment. Trypsin treatment increased the isolation of fungi from clinical samples, and neutral red staining was able to distinguish viable fungal elements.engCandida --isolation & purificationColoring Agents --diagnostic useDermatomycoses --diagnosisEpidermophyton --isolation & purificationHumansMicroscopy --methodsNeutral RedTinea --diagnosisTissue Culture TechniquesTrichophyton --isolation & purificationTrypsin --diagnostic useComparative Study