Sangsiriwut, KAuewarakul, PSuwanagool, SWasi, C2009-05-272009-05-272001-03-10Sangsiriwut K, Auewarakul P, Suwanagool S, Wasi C. Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay. Asian Pacific Journal of Allergy and Immunology. 2001 Mar; 19(1): 43-8http://imsear.searo.who.int/handle/123456789/37225Published by the Allergy and Immunology Society of Thailand.A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count range of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 10(6) CD4+ T-cells. The copy numbers correlated inversely with the CD4 counts.engCD4 Lymphocyte CountCD4-Positive T-Lymphocytes --immunologyDNA, Viral --bloodEndpoint DeterminationHIV Infections --bloodHIV-1 --geneticsHumansPolymerase Chain Reaction --methodsSensitivity and SpecificityThailandComparative Study