Chowdhury, M RMathur, RPrabhakara, KSingh, GDubey, SVerma, I C1996-10-012009-05-271996-10-012009-05-271996-10-01Chowdhury MR, Mathur R, Prabhakara K, Singh G, Dubey S, Verma IC. Improved method for study of chromosomes of chorionic villus samples. Indian Journal of Medical Research. 1996 Oct; 104(): 269-72http://imsear.searo.who.int/handle/123456789/20993Chorionic villus sampling (CVS) offers rapid prenatal diagnosis of chromosomal disorders. We evaluated four methods for chromosomal analysis of chorionic villi (three based on direct preparations and one on long-term culture) in order to define a method which would provide good quality metaphases in sufficient numbers. The direct culturing method using synchronisation and dissociation with dispase was as good as long-term culturing. The optimal period of gestation for study was 10-12 wk.engChorionic Villi SamplingChromosome MappingCytogenetics --methodsEvaluation Studies as TopicFemaleHumansPregnancyTime FactorsJournal Article