Raj, S KSrivastava, AChowdhury, M RJohri, J K2009-05-282009-05-282003-03-23Raj SK, Srivastava A, Chowdhury MR, Johri JK. Association of cucumovirus and potyvirus with betelvine (Piper betle L.) as evidenced by ELISA and RT-PCR. Indian Journal of Experimental Biology. 2003 Mar; 41(3): 273-6http://imsear.searo.who.int/handle/123456789/60032An attempt was made to detect various viruses of Piper betle grown at Mahoba and Banthara in India. DAC-ELISA and RT-PCR tests were performed in leaf sap samples of betelvine for detection of a cucumovirus (Cucumber mosaic virus) and potyvirus (Bean yellow mosaic virus) using specific antibodies and universal primers of respective viruses. DAC-ELISA could detect only CMV. However, RT-PCR detected both cucumovirus and potyvirus infection in betelvine samples. Association of CMV with betelvine was observed for the first time in the present study.engAntibodies, Viral --analysisBase SequenceCucumovirus --geneticsDNA PrimersDNA, Viral --analysisEnzyme-Linked Immunosorbent AssayPiper betle --virologyPotyvirus --geneticsReverse Transcriptase Polymerase Chain ReactionJournal Article