Velineni, SAsuthkar, SUmabala, PLakshmi, VSritharan, M2007-01-232009-05-282007-01-232009-05-282007-01-23Velineni S, Asuthkar S, Umabala P, Lakshmi V, Sritharan M. Serological evaluation of leptospirosis in Hyderabad Andhra Pradesh: a retrospective hospital-based study. Indian Journal of Medical Microbiology. 2007 Jan; 25(1): 24-7http://imsear.searo.who.int/handle/123456789/53812PURPOSE: Leptospirosis is a zoonotic disease with humans getting the infection either from rodent hosts or from domestic animals. Urine contaminated environment is the common source of infection. This is an under-reported disease in Andhra Pradesh. We report a retrospective hospital-based study on 55 patients with suspected leptospirosis. METHODS: A total of 55 serum samples were collected from patients with suspected leptospirosis and subjected to serological testing by LeptoTek Dri-dot, microscopic agglutination test (MAT) and IgM enzyme-linked immunosorbent assay (ELISA). Identification of the predominant infecting serotype was done using a panel of 12 serovars. RESULTS: MAT analysis of all the 55 samples identified all cases to be positive. The predominant serogroup was Icterohaemorrhagiae (68%) followed by Australis (22%), Autumnalis (8%) and Javanica (2%). LeptoTek Dri-dot showed a sensitivity of 96% as compared to MAT. IgM ELISA done on 32 samples showed a sensitivity of 86.7% compared to MAT. CONCLUSIONS: MAT helped to identify Icterohemorrhagiae as the predominant serovar in this study. Despite the small number of samples analyzed, the data obtained establishes a need for a prospective study in this region.engCells, CulturedEnzyme-Linked Immunosorbent AssayHemagglutination TestsHospitalsHumansImmunoglobulin M --immunologyIndiaLeptospira --growth & developmentLeptospirosis --bloodRetrospective StudiesSerologic TestsJournal Article