Banerjee, D KVendrell-Ramos, M1993-12-012009-05-271993-12-012009-05-271993-12-01Banerjee DK, Vendrell-Ramos M. Is asparagine-linked protein glycosylation an obligatory requirement for angiogenesis? Indian Journal of Biochemistry & Biophysics. 1993 Dec; 30(6): 389-94http://imsear.searo.who.int/handle/123456789/27515Dependence of protein N-glycosylation on capillary endothelial cell proliferation has been studied. Amphomycin, a potent N-glycosylation inhibitor, inhibited capillary endothelial cell proliferation in a dose-dependent manner. beta-Agonist isoproterenol as well as other intracellular cAMP enhancing agents, viz. cholera toxin, prostaglandin E1 and 8Br-cAMP, also enhanced capillary endothelial cell proliferation. In addition to cell proliferation, isoproterenol also enhanced protein glycosylation in these cells. Isoproterenol effect was mediated by beta-adrenoreceptors, as it got reduced on pre-treatment of cells with either atenolol or ICI 118, 551 or propranolol. Furthermore, isoproterenol stimulation of protein glycosylation by exogenous dolichyl monophosphate and its inhibition by tunicamycin (GlcNAc-1P transferase inhibitor) supported the concept that isoproterenol specifically stimulated protein N-glycosylation event(s) in the cell.eng8-Bromo Cyclic Adenosine Monophosphate --pharmacologyAdrenal Medulla --cytologyAdrenergic beta-Antagonists --pharmacologyAlprostadil --pharmacologyAnimalsAnti-Bacterial Agents --pharmacologyAsparagineCattleCell Division --drug effectsCells, CulturedCholera Toxin --pharmacologyCyclic AMP --metabolismEndothelium, Vascular --cytologyGlycosylation --drug effectsIsoproterenol --pharmacologyLipopeptidesNeovascularization, PathologicOligopeptides --pharmacologyProtein Processing, Post-Translational --drug effectsJournal Article