Kubo, TRai, S KRawal, SYamano, T2009-05-272009-05-271993-12-01Kubo T, Rai SK, Rawal S, Yamano T. Serological study of Japanese encephalitis in Nepal. The Southeast Asian Journal of Tropical Medicine and Public Health. 1993 Dec; 24(4): 756-61http://imsear.searo.who.int/handle/123456789/35528The Southeast Asian Journal of Tropical Medicine and Public Health.Antibodies against Japanese encephalitis virus (JEV) were examined in 356 serum samples from individuals visiting Tribhuvan University Teaching Hospital using neutralization test. A total of 13.2% (47/356) seropositivity was observed. Antibody positivity against both Nakayama-NIH and Beijing-1 strains was seen in 7.9% subjects. Infection by Nakayama-NIH strain alone was seen to be 4.2% while 1.1% of study populations were found to be infected only by Beijing-1 strain. High antibody titer was observed in the 20-40 years age-group and did not increase with age. Seropositivity was more common in zone-A (Tarai) area compared to zone-B (Hills) and zone-C (Mountains). Spectrum of antigenicity of JEV in zone-A as shown by neutralization test was identical to Indian isolates (strains). Difference in seropositivity was observed against Nakayama-NIH and Beijing-1 strain in zone-B and C indicating recent spread of JEV in these areas.engAdolescentAdultAgedAntibodies, Viral --isolation & purificationEcologyEncephalitis Virus, Japanese --immunologyEncephalitis, Japanese --epidemiologyFemaleHumansMaleNepal --epidemiologyNeutralization TestsSeroepidemiologic StudiesJournal Article