Ismail, Ahmad Fahmi HarunDoolaanea, Abd AlmonemMohamed, FarahidahMansor, Nur 'IzzatiShafri, Mohd Affendi MohdYusof, Fathin Athirah2015-11-282015-11-282015-09Ismail Ahmad Fahmi Harun, Doolaanea Abd Almonem, Mohamed Farahidah, Mansor Nur 'Izzati, Shafri Mohd Affendi Mohd, Yusof Fathin Athirah. Method Development and Validation using UV Spectrophotometry for Nigella sativa Oil Microparticles Quantification. Journal of Applied Pharmaceutical Science. 2015 Sept; 5(9): 82-88.2231-3354http://imsear.searo.who.int/handle/123456789/166458Nigella sativa oil (NSO) has been exploited for medical purposes for many generations. The fabrication of microparticles containing NSO intended for sustained release was done to be used in treating osteomyelitis. Method in quantifying NSO using UV-spectroscopy was developed and validated. Linearity shown a good correlation coefficient with the values higher than 0.995, both for actual and different analysts. The LOD and LOQ values were recorded to be 2.89 μg/mL and 8.75 μg/mL respectively. In addition, the highest %RSD values for the intermediate and repeatability studies were 0.970% and 0.445% which suggested the method was precise. The percentage recovery for 4 known concentrations gave the range between 98.16% to 99.39%, indicating the high accuracy of the method. The parameters analyzed in this study were in accordance with ICH Q2 (R1) guidelines.enMethod DevelopmentMethod ValidationN. sativaMicroparticlesArticle