Hu, MKirinoki, MYokoi, HKawai, SChigusa, YMatsuda, H2009-05-272009-05-271999-03-01Hu M, Kirinoki M, Yokoi H, Kawai S, Chigusa Y, Matsuda H. Human antibody isotype responses to Schistosoma japonicum egg antigen. The Southeast Asian Journal of Tropical Medicine and Public Health. 1999 Mar; 30(1): 24-8http://imsear.searo.who.int/handle/123456789/35475The Southeast Asian Journal of Tropical Medicine and Public Health.Schistosoma japonicum-infected subjects from Hubei province of China were investigated to determine the class and subclass of the antibody response to soluble egg antigen (SEA), using an enzyme-linked immunosorbent assay. The subjects were 50 acute and 55 chronic cases. In acute cases, the mean OD values for IgA, IgE and IgG3 were very high, while the positive ratios of IgA and IgE were only 78% and 74%, respectively. The positive ratios of IgG, IgM, IgG1, IgG3 and IgG4 were all above 90%. In chronic cases, the mean OD values for IgG, IgG3 and IgG4 were very high, and the positivity rates of IgG, IgG1, IgG3 and IgG4 were all above 90%. Comparing the two study groups, the mean OD values of IgM, IgA, IgE were higher in acute cases than those of chronic cases (p < 0.0001), while the mean OD values of IgG, IgG4 were higher in chronic cases than in acute cases (p < 0.05). The mean OD values of IgG3 in both groups were high and those of IgG2 in both groups were low.engAcute DiseaseAdolescentAdultAnimalsAntigens, Helminth --diagnostic useCase-Control StudiesChildChronic DiseaseEnzyme-Linked Immunosorbent Assay --methodsHumansImmunoglobulin A --bloodImmunoglobulin E --bloodImmunoglobulin G --bloodImmunoglobulin M --bloodMiddle AgedOvum --immunologyReproducibility of ResultsSchistosoma japonicum --immunologySchistosomiasis japonica --bloodSensitivity and SpecificityComparative Study