Varghese, SShameena, BSudhakaran, P ROommen, O V2009-05-282009-05-282001-05-21Varghese S, Shameena B, Sudhakaran PR, Oommen OV. Short-term effects of thyroid hormones on lipogenic enzymes and 14C-acetate incorporation into various lipid classes: in vivo and in vitro studies. Indian Journal of Experimental Biology. 2001 May; 39(5): 431-5http://imsear.searo.who.int/handle/123456789/57310Short-term effect of 3,5,3'-triiodothyronine (T3) and 3,5-diiodothyronine (T2) on lipid metabolism in the liver of Anabas testudineus was examined. In vivo injections of both T3 and T2 at a concentration of 10 ng/g body weight increased malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) activity compared to 6-propylthiouracil (6-PTU) treated group. Treatment of 6-PTU results in the accumulation 14C-acetate into fat and thyroid hormones' treatment reduce it. In vitro experiments show that malic enzyme activity is augmented only by high concentration of T3 (10(-7) M) where as all concentrations of T2 increase its activity. In vitro studies with T3 showed a biphasic effect on cholesterol content. Conversely T2 in vitro, reduced cholesterol content with all concentrations. From these results it can be concluded that both T3 and T2 have short-term effect on lipid metabolism in Anabas.engAcetic Acid --metabolismAnimalsCholesterol --metabolismDiiodothyronines --pharmacologyFemaleGlucosephosphate Dehydrogenase --metabolismIsocitrate Dehydrogenase --metabolismLipid MetabolismMalate Dehydrogenase --metabolismMalate Dehydrogenase (NADP+)Perciformes --metabolismPhosphogluconate Dehydrogenase --metabolismThyroid Hormones --pharmacologyTriiodothyronine --pharmacologyIn Vitro