Kaur, RavneetFilia, GursimranKaur, PaviterLeishangthem, Geeta Devi2020-10-162020-10-162019-02Kaur Ravneet, Filia Gursimran, Kaur Paviter, Leishangthem Geeta Devi. Comparative Evaluation of Serological Tests for Diagnosis of Brucellosis in Bovines with History of Abortion in Punjab. Journal of Animal Research. 2019 Feb; 9(1): 67-732249-66292277-940Xhttp://imsear.searo.who.int/handle/123456789/210879Brucellosis is highly infectious zoonotic disease that causes huge economic losses to livestock farmers by affecting the reproductive potential of animals causing last trimester abortions and infertility. In the present study evaluation of different serological tests to diagnose the seroprevalence of brucellosis in bovines with history of abortion using various serological tests [Rose Bengal Plate Test (RBPT), modified rose bengal plate test (mRBPT), microtitre plate agglutination test (MAT) and indirect enzyme linked immunosorbent assay (i-ELISA)] was carried out. A total of 134 blood samples of cattle and buffalo with history of abortion were collected from organized and unorganized farms. Seroprevalence by mRBPT, RBPT, MAT and i-ELISA was 75.37%, 67.91%, 72.38% and 72.38%, respectively. In organized farms, prevalence of 78.12%, 81.25%, 78.12% and 81.25% while in unorganized farms prevalence of 64.70%, 73.52%, 70.58% and 69.60% was reported by RBPT, mRBPT, MAT and i-ELISA, respectively. The sensitivity and specificity of serological tests by keeping i-ELISA as gold standard were also calculated and the results revealed that sensitivities of RBPT, mRBPT and MAT were 91.75%, 97.94% and 96.91%, respectively, whereas specificities were 94.59%, 83.78% and 91.89%, respectively.Bovine Brucellosisi-ELISAMATmRBPTRBPTJournal ArticleIndiaDepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana Punjab, INDIAAnimal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, INDIADepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana Punjab, INDIAAnimal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, INDIA