Ray, RDas, A KDutta, N KChakrabarty, A NChaudhuri, B NSeth, SDastidar, Sujata G2009-05-282009-05-282002-02-08Ray R, Das AK, Dutta NK, Chakrabarty AN, Chaudhuri BN, Seth S, Dastidar SG. Potentiality of a new compound for in vitro differentiation between halophilic and non-halophilic vibrios. Indian Journal of Experimental Biology. 2002 Feb; 40(2): 220-2http://imsear.searo.who.int/handle/123456789/59323Sensitivity of 21 halophilic vibrios and 16 clinical isolates of non-halophilic vibrios was determined against a new possible antivibrio agent, a pyrimidine analogue, 4, 6-dimethylpyrimidine -2-thiol (4,6-DMPT). It appeared to be a vibriocidal agent, having a mean MIC and MBC of 32 microg/ml for halophilic strains and 64 microg/ml for non-halophilic strains and an LD50 of 300 mg/Kg body weight of mice. Thus, 4,6-DMPT may help an in vitro distinction between halophilic and non-halophilic vibrios. Sensitivity of these strains was also studied with respect to pteridine, crystal violet and Tween 80 hydrolysis as further markers distinguishing between these 2 groups which could also be differentiated by their growth on TCBS or/and CLED media.engAnimalsAnti-Bacterial Agents --pharmacologyAnti-Infective Agents, Local --pharmacologyGentian Violet --pharmacologyHydrolysisMiceMicrobial Sensitivity TestsPolysorbates --pharmacologyPteridines --pharmacologyPyrimidines --pharmacologySensitivity and SpecificitySurface-Active Agents --pharmacologyVibrio cholerae --classificationVibrio parahaemolyticus --classificationJournal Article