Imwidthaya, PMieskes, KRienthong, S2009-05-272009-05-272001-06-15Imwidthaya P, Mieskes K, Rienthong S. Evaluation of katG codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from Thailand. Journal of the Medical Association of Thailand. 2001 Jun; 84(6): 864-9http://imsear.searo.who.int/handle/123456789/40645Chotmaihet Thangphaet.Nine isoniazid (INH)-susceptible and 11 INH-resistant Mycobacterium tuberculosis clinical isolates were analyzed for katG codon 315 mutations by polymerase chain reaction (PCR) assay using primers MYC-32 and MYC-33, followed by restriction fragment length polymorphism. After AciI digestion of PCR products, all 9 INH-susceptible isolates and 5 out of 11 (45%) INH-resistant isolates showed 0.12 kb band, which was previously reported to indicate wild type, whereas, 6 of 11 (55%) INH-resistant isolates lacked this band.engAntitubercular Agents --pharmacologyBacterial ProteinsDrug Resistance, MicrobialHumansIsoniazid --pharmacologyMutationMycobacterium tuberculosis --drug effectsPeroxidases --geneticsPolymerase Chain ReactionJournal Article