Nayak, B RamakrishnaShenoy, B CPattabiraman, T N2015-07-172015-07-171979-03Nayak B Ramakrishna, Shenoy B C, Pattabiraman T N. Studies on plant gums. Role of calcium in polysaccharide-protein interaction in the neem (Azadirachta indica) gum. Journal of Biosciences. 1979 Mar; 1(1): 61-68.http://imsear.searo.who.int/handle/123456789/159927The partial removal of tightly bound Ca2+ from dialysed neem (Azadirachta indica) gum, resulted in the release of a basic protein from a highly anionic polysaccharide-protein complex as evidenced by chromatographic studies on TEAEcellulose. Complete removal of Ca2+ caused, in addition, the release of a minor heteropolysaccharide which was found in association with the basic protein. These processes were reversed on the addition of Ca2+. The gum, in addition, contained a protein-rich component accounting for 35% protein and 7ยท5% total carbohydrate. This component behaved as a distinct entity during ion-exchange chromatography of the native gum solutions, or which were either partially or completely depleted of bound Ca2+.enNeemAzadirachta indica gumcalciumbasic proteinpolysaccharideprotein interactionsStudies on plant gums. Role of calcium in polysaccharide-protein interaction in the neem (Azadirachta indica) gum.Article