Subramaniam, GPalasubramaniam, SNavaratnam, P2006-07-172009-05-282006-07-172009-05-282006-07-17Subramaniam G, Palasubramaniam S, Navaratnam P. SHV-5 extended-spectrum beta-lactamases in clinical isolates of Escherichia coli in Malaysia. Indian Journal of Medical Microbiology. 2006 Jul; 24(3): 205-7http://imsear.searo.who.int/handle/123456789/53929Escherichia coli isolates resistant to ceftazidime isolated in the University Malaya Medical Center (UMMC) Kuala Lumpur, Malaysia, between the years 1998 and 2000 were studied for extended-spectrum beta-lactamase (ESBL) production. All strains were analysed phenotypically and genotypically and found to be ESBL-producing organisms harbouring SHV-5 beta-lactamase. This was confirmed by PCR-SSCP and nucleotide sequencing of the blaSHV amplified gene. As there was no evidence of ESBL activity in E. coli prior to this, coupled with the fact that there was a predominance of SHV-5 beta-lactamases in Klebsiella pneumoniae isolates in UMMC, we postulate that the E. coli obtained the SHV-5 beta-lactamase genes by plasmid transfer from the ESBL-producing K. pneumoniae.engAnti-Bacterial Agents --pharmacologyCeftazidime --pharmacologyDNA, BacterialDrug Resistance, BacterialEscherichia coli --drug effectsEscherichia coli Infections --microbiologyGene Transfer, HorizontalGenotypeHumansKlebsiella pneumoniae --geneticsMalaysiaMicrobial Sensitivity TestsPhenotypePolymerase Chain ReactionPolymorphism, Single-Stranded Conformationalbeta-Lactam Resistancebeta-Lactamases --geneticsJournal Article