Zaidi, S N AShipstone, A CGarg, N K2015-07-172015-07-171979-03Zaidi S N A, Shipstone A C, Garg N K. Effect of phospholipase-D on rat kidney mitochondria. Journal of Biosciences. 1979 Mar; 1(1): 75-82.http://imsear.searo.who.int/handle/123456789/159932Incubation of purified rat kidney mitochondrial fraction with phospholipase- D resulted in the accumulation of phosphatidic acid in the membrane due to the degradation of membrane-bound phosphatidylcholine, -serine and -ethanolamine Simultaneously with the hydrolysis of the phospholipids, cholesterol and protein were released from the mitochondrial membrane into the medium, and binding of Ca2+ by mitochondrial membranes increased. Phospholipase Dtreated mitochondrial fraction exhibited increased swelling in vitro in the early stages of incubation (15 min) after which the mitochondria were ruptured. Membrane- bound adenosine triphosphatase was partially inactivated and the enzyme activity was not significantly restored by incubation with sonicated dispersions of phosphatidylcholine, -serine and cholesterol. These results indicate that removal of choline, serine and ethanolamine from membrane-bound phospholipids disrupt phospholipid-cholesterol and phospholipid-protein association and affect functions of the membrane.enMitochondriaphospholipase-Dphosphatidylcholinephosphatidylethanolaminephosphatidylserineswellingadenosine triphosphataseCa2+ bindingArticle