Oz Oyar, EserYildirim, ZuhalPampal, ArzuIlhan, Ayse SebnemLortlar Unlu, Nese2023-08-092023-08-092022-08Oz Oyar Eser, Yildirim Zuhal, Pampal Arzu, Ilhan Ayse Sebnem, Lortlar Unlu Nese. Local and systemic effects of adrenomedullin after intestinal ischemia reperfusion. Indian Journal of Experimental Biology. 2022 Aug; 60(8): 580-5860975-10090019-5189http://imsear.searo.who.int/handle/123456789/222519Adrenomedullin (AM) is a peptide proven to increase cellular tolerance to hypoxia and oxidative stresss and contribute angiogenesis. Despite its known therapeutic effects on myocardial, renal or spinal ischemic reperfusion injuries, its local and systemic effects on intestinal ischemic reperfusion injury still remain unknown. This study aims to demonstrate the local and systemic effects of AM on Intestinal Ischemic Reperfusion Injury (I-IRI) demonstrated in rats. Thirty male rats were randomly allocated to five groups: Control, Adrenomedullin (AM), Intestinal Ischemic Reperfusion Injury (I-IRI), Adrenomedullin and Intestinal Ischemic Reperfusion Injury (AM+I-IRI), and Intestinal Ischemic Reperfusion Injury and Adrenomedullin (I-IRI+AM). Blood and tissue samples were obtained for biochemical and histopathological evaluation. The results were expressed as mean±SEM and, P <0.05 was considered statistically significant. The levels of inflammatory cytokines were found to be elevated in I-IRI group and depleted in I-IRI+AM group. The biochemical and histopathological markers of injuries at the intestine and remote organs were found to be recuperated when the AM applied before the reperfusion phase. Results of this study demonstrated that the therapeutic drug adrenomedullin (AM) could reverse the intestinal and remote organ injuries related to intestinal ischemic reperfusion injury (I-IRI). These effects might be related to the antioxidant, anti-inflammatory, and anti-apoptotic activities of AM.InflammationIntestinal ischemic reperfusion injuryOxidative stressJournal ArticleIndiaDepartment of Physiology, Faculty of Medicine, Katip Celebi University, 35640 Cigli, Izmir, TurkeyEtimesgut Public Health Laboratory, 06770 Etimesgut, Ankara, TurkeyDepartment of Pediatric Surgery, Faculty of Medicine, Ufuk University, 06520 Balgat, Ankara, TurkeyDepartment of Basic Medical Sciences, Gulhane Faculty of Dentistry, University of Health Sciences, 06010 Etlik, Ankara, TurkeyDepartment of Biomedical Engineering, Boston University, Boston, USA