Hong, HeXueting, WangCongcong, LiXiaotong, DengYan, FanJianxin, Han2020-10-162020-10-162020-05Hong He, Xueting Wang, Congcong Li, Xiaotong Deng, Yan Fan, Jianxin Han. Hyphal Formation is Enhanced by 121°C Heat Inactivation of Serum Added to Culture Media. Journal of Advances in Medicine and Medical Research. 2020 May; 32(6): 12-202456-8899http://imsear.searo.who.int/handle/123456789/210360Background: A novel method for inducing hyphae formation, was described.Methods:Mycelia of experimental and clinical strains from 100 patients with oral lichen planuswere cultured in RPMI 1640 medium with 56°C or 121°C inactivated calf sera. After these strains were cultured for 2 h to 7 days, the average hyphae formation rate was observed under light microscopy. Results: The 121°C inactivated calf serum showed better outcomes than 56°C inactivated calf serum in terms of time and amounts of hyphae products. Conclusions:For Candida albicanshyphae culture, the 121°C inactivated serum is superior to the 56°C inactivated serumSerum inactivationCandida albicanshyphaeyeastoral lichen planusJournal ArticleIndiaAffiliated Stomatology Hospital, School of Medicine, Zhejiang University,395 Yan’an Road, Hangzhou, 310006, ChinaKey Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, ChinaAffiliated Stomatology Hospital, School of Medicine, Zhejiang University,395 Yan’an Road, Hangzhou, 310006, ChinaKey Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, ChinaAffiliated Stomatology Hospital, School of Medicine, Zhejiang University,395 Yan’an Road, Hangzhou, 310006, ChinaKey Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou, ChinaHangzhou StomatologyHospital, Pinghai Road, Hangzhou, 310000, ChinaDepartment of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang University, 310058, China