Ketupanya, AphornpiromAranyakasemsuk, NiwatTocharoentanaphol, ChintanaVuthiwong, Chutawadi2009-05-272009-05-272005-11-25Ketupanya A, Aranyakasemsuk N, Tocharoentanaphol C, Vuthiwong C. Chromosome analysis of uncultured amniocytes by comparative genomic hybridization in early amniocentesis. Journal of the Medical Association of Thailand. 2005 Nov; 88 Suppl 3(): S1-6http://imsear.searo.who.int/handle/123456789/39531Chotmaihet Thangphaet.OBJECTIVE: To study chromosome analysis by comparative genomic hybridization (CGH) compared with the conventional technique in early amniocentesis. MATERIAL AND METHOD: Cross-sectional descriptive study design was performed in 32 singleton pregnant women with gestational age between 12-15 weeks. Transabdominal amniocentesis was carried out under ultrasound guidance. The amniotic fluid samples were simultaneously investigated using CGH and the conventional cytogenetics study as a gold standard. RESULTS: Amniocentesis were done for advanced maternal age in all cases. The mean maternal age was 35.8 years (35-42 years). The mean gestational age was 13.7 weeks (12-15 weeks). The chromosome analysis by CGH technique of uncultured amniocyte showed 17 normal female chromosomes (53.1%) and 15 normal male chromosomes (46.9%). This finding was the same as the conventional cytogenetics method. The mean duration of the CGH method was 6 days and that of the conventional cytogenetics method was 13.7 days (10-23 days). CONCLUSION: The CGH technique is a reliable technique for a rapid prenatal diagnosis of chromosome study in early gestation.engAdultAmniocentesisAmniotic Fluid --cytologyChromosome AberrationsFemaleHumansKaryotyping --methodsMaleNucleic Acid HybridizationPregnancyReproducibility of ResultsClinical Trial