Shukla, AvanishMuhammed, Imtiaz KhalidSheshala, RaviMhaisker, Ibrahim UsmanRampal, Krishna2020-10-162020-10-162020-07Shukla Avanish, Muhammed Imtiaz Khalid, Sheshala Ravi, Mhaisker Ibrahim Usman, Rampal Krishna. Acute toxicity evaluation of homeopathic preparation of Gymnema sylvestre and analysis of its chemical constituents. Journal of Applied Biology & Biotechnology. 2020 Jul; 2020 Jul: 33-372455-70052347-212Xhttp://imsear.searo.who.int/handle/123456789/209917In many countries, homeopathic preparation is believed to be an effective medicine for various ailments.However, there are limited scientific evidences in regard to its usage, safety, and efficacy. It is necessary toupdate this age-old scientific wisdom in different aspects, including pharmacologic and therapeutic potentials.In this study, we assessed the safety profile of homeopathic preparation of Gymnema sylvestre (HPGS). Itschemical constituents were deciphered using LCMS approaches. HPGS was subjected to an acute toxicitystudy (OECD-423 guidelines) using Sprague Dawley rats. The administration of HPGS did not produce anytoxic symptoms or show mortality at the dose level of 300 mg/kg body weight. Phytochemical analysis revealedthat HPGS contained alkaloids, saponins, and flavonoids. These results demonstrated the non-toxic nature ofHPGS in vivo, suggesting a long-term usage in clinical practices when administered orallyGymnema sylvestrehomeopathytoxicityLC/MSSprague Dawley ratsAcute toxicity evaluation of homeopathic preparation of Gymnema sylvestre and analysis of its chemical constituentsJournal ArticleIndiaFaculty of Traditional and Complementary Medicine, Cyberjaya University College of Medical Science, Cyberjaya, MalaysiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Cyberjaya University College of Medical Science, Cyberjaya, MalaysiaFaculty of Pharmacy, University Teknologi MARA UiTM, Puncak Alam, MalaysiaFaculty of Traditional and Complementary Medicine, Cyberjaya University College of Medical Science, Cyberjaya, MalaysiaCentre for Graduate Studies, Research and Commercialization, Cyberjaya University College of Medical Science, Cyberjaya, Malaysia