Khantisitthiporn, OnruedeeSookrung, NitatTungtrongchitr, AnchaleeTongtawe, PongsriBunnag, ChaweewanSrimanote, PotjaneeTapchaisri, PramuanChaicumpa, Wanpen2009-05-272009-05-272007-12-12Khantisitthiporn O, Sookrung N, Tungtrongchitr A, Tongtawe P, Bunnag C, Srimanote P, Tapchaisri P, Chaicumpa W. Native troponin-T of the American cockroach (CR), Periplaneta americana, binds to IgE in sera of CR allergic Thais. Asian Pacific Journal of Allergy and Immunology. 2007 Dec; 25(4): 189-97http://imsear.searo.who.int/handle/123456789/36624Published by the Allergy and Immunology Society of Thailand.The American cockroach, Periplaneta americana, is the predominant cockroach (CR) species in Thailand and a major source of indoor allergens second only to the house dust mite. The incidence of CR allergy among allergic Thai patients is increasing but basic information on the allergenic components is scarce. In this study a recombinant troponin-T was produced by using cDNA prepared from RNA of the P. americana as a template and PCR primers designed from the P. americana troponin-T sequence deposited in the GenBank database. The recombinant protein (Mr approximately 50) did not bind to IgE in the sera of 18 skin prick test positive CR allergic patients. Rabbit polyclonal antiserum (PAb) against the recombinant troponin-T was produced and used in preparing an affinity column for the purification of native troponin-T from the crude P. americana extract (Mr approximately 47). IgE-immunoblotting revealed that the native protein bound to IgE in 3 of the 18 (16.7%) patients. Our results imply that native P. americana troponin-T, but not its recombinant counterpart, is a minor allergen among the CR allergic Thais.engAir Pollution, IndoorAllergens --immunologyAnimalsFemaleHumansHypersensitivity --bloodImmunoglobulin E --bloodInsect Proteins --immunologyMalePeriplaneta --immunologyPyroglyphidae --immunologyRecombinant Proteins --immunologyThailandTroponin T --immunologyJournal Article