Sheela, PAmuthan, GMahadevan, A2009-05-282009-05-281996-01-01Sheela P, Amuthan G, Mahadevan A. Cloning of extracellular lipase gene from Xanthomonas campestris pathovar sesami on to Escherichia coli. Indian Journal of Experimental Biology. 1996 Jan; 34(1): 27-31http://imsear.searo.who.int/handle/123456789/60072A lipase gene from X. campestris pv. sesami (strain XcS 1) causal agent of leaf spot disease of Sesamum indicum, was cloned onto E. colt. XcS showed the presence of lip+ transformants on Dye's medium with 1% glycerol as sole carbon source. The recombinant plasmids were isolated and when digested with Eco R1, yielded 2 fragments with molecular weights 4.0 and 4.8 kb. Thus a 8.8 kb insert DNA fragment was obtained which showed lipase activity.engCloning, MolecularEscherichia coli --geneticsGenes, BacterialLipase --geneticsXanthomonas campestris --enzymologyJournal Article