Saoji, A MNaghi, T1989-07-012009-05-291989-07-012009-05-291989-07-01Saoji AM, Naghi T. Identification of C-3 on polyacrylamide gel by pre-staining the normal human serum with Remazol Brilliant Blue and raising monospecific anti C-3 antibody. Indian Journal of Pathology & Microbiology. 1989 Jul; 32(3): 221-6http://imsear.searo.who.int/handle/123456789/74180Based on our earlier work we hereby describe pre-staining of normal human serum with Remazol Brilliant Blue (RBB) to precisely locate the third component of complement system (C3,B1C) on polyacrylamide gels. Testing the dye-protein complex eluted from different immunoreactive post-transferrin (origin to transferrin) components against the standards anti-C3 antibody in various gel precipitation techniques, revealed that the prominent band just posterior to the centrally placed transferrin is of complement. Subsequent to its identity, we also evolved a method to raise an anti-C3 antibody. The resulting antibody was found to be monospecific when assessed in different gel precipitation procedures.engAnthraquinonesAntibodiesColoring AgentsComplement C3 --isolation & purificationElectrophoresis, Polyacrylamide GelHumansJournal Article