Bhattacharyya, DipankarBasu, ArindamSen, Parimal C2007-04-012009-05-272007-04-012009-05-272007-04-01Bhattacharyya D, Basu A, Sen PC. Purification of protein from a crude mixture through SDS-PAGE transfer method. Indian Journal of Biochemistry & Biophysics. 2007 Apr; 44(2): 122-5http://imsear.searo.who.int/handle/123456789/26698SDS-polyacrylamide gel electrophoresis (SDS-PAGE) transfer method was used for purification and enrichment of the protein from crude sample. Coomassie bluc/ZnSO4 stained protein band(s) containing intact polyacrylamide gel were loaded on to another polyacrylamide gel either alone or as pooled gel bands. Two/three bands were combined together and arranged tightly over one another, sealed with stacking gel and ran in another gel, which was quite useful for enrichment and purification of a particular protein from a complex mixture. Recovery of protein by gel transfer method was found to be 70% in case of ZnSO4 staining, whereas around 30% recovery was possible, following Coomassie blue staining. The method described here for purification of protein(s) from a complex mixture, following gel transfer procedure could be useful for further characterization of the desired protein.engAnimalsCattleElectrophoresis, Polyacrylamide GelGoatsMaleSerum Albumin, Bovine --isolation & purificationSpermatozoa --chemistryStaining and LabelingTestis --chemistryJournal Article