Bansal, O P2016-03-162016-03-162015Bansal O P. Effects of Tetracycline Son Soil Enzyme Activities in an Alluvial Soil. British Journal of Medicine and Medical Research. 2015; 5(8): 1007-1016.http://imsear.searo.who.int/handle/123456789/176008Aims: To study the effect of various doses of tetracycline (TC), oxytetracycline (OTC) and chlortetracycline (CTC) on the enzymatic (DHA, acid phosphatase, alkaline phosphatase, urease and catalase) activity in sewage amended and un-amended alluvial soil. Methodology: A laboratory incubation study was conducted during 2012-2013 on an Aligarh farm alluvial soil. The enzymatic activity was studied in presence of various doses of three tetracycline antibiotics in soil; soil amended with sewage sludge; and mixture of sewage sludge and tetracyclines at different time intervals [(0 (4h), 7, 14, 21, 35, 56, 70,91d]. Results: Activity of all the studied enzymes was significantly inhibited for up to 14-21 days of incubation (14 d for DHA and acid phosphatase, 21 d for alkaline phosphatase, urease and catalase) and thereafter inhibition got weaker. The activity of all the studied enzymes decreased with increase in the doses of tetracyclines. Higher enzymatic activity was observed in sewage sludge amended soil than in the un-amended soil. Tetracyclines in presence of sewage sludge were found to have no appreciable effect on enzymatic activities. Conclusions: Dehydrogenase, acid and alkaline phosphatase, urease and catalase activity in tetracycline free soil was superior to soil containing antibiotics in the period of 2-3 weeks of incubation. The inhibition of soil enzyme activity was directly proportional to tetracycline concentration. In presence of sewage sludge, the studied enzymes activity initially increased up to 3 weeks and decreased thereafter. In presence of sewage sludge and antibiotics, the activity of enzymes remains almost unchanged. The activity of studied enzymes in soil was positively correlated to soil organic content.enAntibioticstetracyclineoxytetracyclinechlortetracyclinesewage sludgesoil enzyme activityArticle