Deshpande, A SSurendranathan, K KNair, P M1992-10-012009-05-271992-10-012009-05-271992-10-01Deshpande AS, Surendranathan KK, Nair PM. Identification of a non-microsomal cinnamic acid 4-hydroxylase from potato tuber (S. tuberosum) and its partial purification. Indian Journal of Biochemistry & Biophysics. 1992 Oct; 29(5): 418-24http://imsear.searo.who.int/handle/123456789/27787The cytoplasmic localisation of cinnamic acid 4-hydroxylase (CA4H) has been shown by isolation and subcellular fractionation of the enzyme in Hepes buffer. The enzyme was purified by ammonium sulphate fractionation followed by AcA-34 molecular sieve chromatography. The enzyme existed as a high molecular mass which dissociated to a lower form on dilution on the column. The pH optimum, sulphydryl requirement, molecular and preliminary kinetic characteristics were investigated.engCations, DivalentCell FractionationCytochrome P-450 Enzyme System --isolation & purificationHydrogen-Ion ConcentrationKineticsMicrosomes --enzymologyMixed Function Oxygenases --isolation & purificationSolanum tuberosum --enzymologySubcellular Fractions --enzymologyTrans-Cinnamate 4-MonooxygenaseJournal Article