Ghosh, TanushreeJena, SwetalinaBehera, Aruna RaniPanigrahy, SangeetaSethi, Rajesh Kumar2023-06-172023-06-172022-08Ghosh Tanushree, Jena Swetalina, Behera Aruna Rani, Panigrahy Sangeeta, Sethi Rajesh Kumar. Microbial Profile of Neonatal Septicemia and its Antibiogram Prevalent in a Tertiary Care Hospital of Western Odisha. Journal of The Indian Medical Association. 2022 Aug; 120(8): 17-220019-5847http://imsear.searo.who.int/handle/123456789/216592Introduction : A disseminated disease with positive Blood Culture during the first month of life and encompasses various systemic infections of the newborn such as septicemia, meningitis, pneumonia, arthritis, osteomyelitis and Urinary Tract Infection is defined as Neonatal Sepsis. It is one of the leading causes of morbidity and mortality amongst neonates of developing countries. Aim : To determine the microbial profile of Blood Culture-positive Septicemia cases and study their antimicrobial susceptibility pattern. Materials and Methods : Blood Culture and C-reactive Protein (CRP) estimation were done for all 220 clinically suspected neonates. All the pure Bacterial and Candida isolates were identified using standard biochemical tests. Antimicrobial susceptibility testing was done for all bacterial isolates using the Kirby-Bauer disk diffusion method as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Results : Out of 220 cases, 68.2% were culture positive. Early-onset Neonatal Septicemia (EONS) cases were 74% and Late-onset Neonatal Septicemia (LONS) 26%. The male to female ratio was 1.9:1. Bacterial cases were 66% and 34% were due to Candida. Gram-negative isolates predominated, with Klebsiella pneumonia being the most common one. In the case of Gram-positive isolates, Staphylococcus aureus was most common. The best overall sensitivity of Gram-negative isolates was to Amikacin (100%), Colistin (100%), and Imipenem (96%). Grampositive isolates reported 100% sensitivity to Vancomycin, Teicoplanin and 97.4% to Linezolid. Conclusion : Gram-negative isolates were the leading cause of Sepsis in our study. Strict antimicrobial stewardship should be implemented to prevent the emergence of multi-drug resistant strains.Neonatal septicemiaBlood cultureCRPAntimicrobial susceptibilityJournal ArticleIndiaMD (Microbiology), Demonostrator, Department of Microbiology, Murshidabad Medical College and Hospital, Berhampore 742101 Corresponding AuthorMD (Microbiology), Assistant Professor, Department of Microbiology, VSS Institute of Medical Science and Research, Burla, Odisha 768017MD (Microbiology), Assistant Professor, Department of Microbiology, Great Eastern Medical School and Hospital, Srikakulam, Andhra Pradesh 532484MD (Microbiology), Assistant Professor, Department of Microbiology, Great Eastern Medical School and Hospital, Srikakulam, Andhra Pradesh 532484MD (Paediatrics), Professor, Department of Paediatrics, Great Eastern Medical School and Hospital, Srikakulam, Andhra Pradesh 532484