Shanta, K SBhat, K GPatil, C SJoglekar, H D1998-06-232009-06-011998-06-232009-06-011998-06-23Shanta KS, Bhat KG, Patil CS, Joglekar HD. Evaluation of laboratory techniques for diagnosis of amoebiasis. Journal of Communicable Diseases. 1998 Jun; 30(2): 103-6http://imsear.searo.who.int/handle/123456789/112148Evaluation of 82 cases of amoebiasis including 4 of liver abscess was done. Microscopy of 62 stool samples revealed trophozoites in 19 (30.6%) and cysts in 8 (12.9%). PVA preserved samples were stained by iron-hamatoxylin and trichrome. Twenty-nine samples (46.7%) were positive by culture on Endamoeba, NIH and Charcoal agar. Additional two culture positive cases were negative by direct microscopy. Three (75%) samples of liver abscess pus were culture positive. Of the sera screened for amoebic antibodies by ELISA, 13 (17.3%) were positive. Stool and blood of healthy controls and 25 gastroenteritis cases were similarly analysed. Of healthy controls one had a high ELISA titre.engAnimalsBlood --parasitologyDysentery, Amebic --diagnosisEntamoeba histolytica --growth & developmentEvaluation Studies as TopicFeces --parasitologyHumansLiver Abscess, Amebic --diagnosisComparative Study