Yoosook, CReutrakul, VSantisuk, TChaichana, SPezzuto, J MDong, YClanton, D JKiser, RStaley, P2009-05-272009-05-271998-12-20Yoosook C, Reutrakul V, Santisuk T, Chaichana S, Pezzuto JM, Dong Y, Clanton DJ, Kiser R, Staley P. A sensitive assay for anti-HIV-1 drug discovery in a biological safety level-2 laboratory. The Southeast Asian Journal of Tropical Medicine and Public Health. 1998 Dec; 29(4): 758-62http://imsear.searo.who.int/handle/123456789/33137The Southeast Asian Journal of Tropical Medicine and Public Health.Studies involving infectious, wild type HIV-1 must be performed under strict BSL-3 practice. We have employed a defective (deltaTat/Rev)MC99 and cloned 1A2 line, ie, mutated HIV-1 and Tat/Rev transfected cells to verify anti-HIV-1 activity in a BSL-2 laboratory. A number of extracts from various parts of 11 species of plants were studied. Results were correlated with those of an anti-HIV-1 reverse transcriptase (RT) assay.engAnti-HIV AgentsBiological Assay --methodsCytoprotection --drug effectsDrug DesignGiant Cells --drug effectsHIV Reverse Transcriptase --antagonists & inhibitorsHumansLaboratory Infection --prevention & controlPlant Extracts --therapeutic useReverse Transcriptase InhibitorsJournal Article