Bhaskar, S UdayGopalaswamy, GRaghu, R2009-05-282009-05-282005-03-09Bhaskar SU, Gopalaswamy G, Raghu R. A simple method for efficient extraction and purification of C-phycocyanin from Spirulina platensis Geitler. Indian Journal of Experimental Biology. 2005 Mar; 43(3): 277-9http://imsear.searo.who.int/handle/123456789/63141Phycocyanin is a major light harvesting accessory pigment of red algae and cyanobacteria. In the light of its many commercial applications in food and pharmaceutical industry, purity of the pigment plays a major role. Pharmaceutical industry demands a highly pure phycocyanin with A620/280 ratio of 4 and food industry a ratio of 2. In the present study phycocyanin was extracted in sodium phosphate buffer (pH 7) after macerating in liquid nitrogen. The crude phycocyanin thus extracted was precipitated with 50% ammonium sulphate, purified by dialysis and finally by gel filtration chromatography. Pure phycocyanin was finally obtained with an A620/A280 value of 4.98.engAlgae, Red --metabolismAmmonium Sulfate --pharmacologyBacterial Proteins --metabolismBiochemistry --methodsChromatography, GelCyanobacteria --metabolismElectrophoresis, Polyacrylamide GelLightNitrogenPhycocyanin --chemistryPlant ExtractsSpectrophotometrySpirulinaJournal Article