Anand Singh, LSuson, TGeeta, Chanu CRanjana, Devi KRebachandra, Singh H2024-09-242024-09-242023-12Anand Singh L, Suson T, Geeta Chanu C, Ranjana Devi K, Rebachandra Singh H. Dengue Serotypes In Manipur –Findings From A Retrospective Analytical Study In A Tertiary Care Hospital. International Journal of Medical Research & Review . 2023 Nov; 11(6): 136-1452320-86862321-127Xhttps://imsear.searo.who.int/handle/123456789/231050Objectives:As a part of an ongoing research programme on vector-borne viral diseases especially Dengue, a retrospective analytical study on the occurrence and distribution of Dengue virus serotype(s) in the state of Manipur – a small state situated in the northeastern region of the Indian subcontinent was carried out at Viral Research & Diagnostic Laboratory (VRDL), Department of Microbiology, Regional Institute of Medical Sciences (RIMS), Imphal which is a tertiary care hospital.Materials & Methods:A total of 914 blood samples from clinically dengue-suspected patients were screened for the presence of Dengue infection by adopting the ELISA (IgM) technique during the period from 01/06/2022 to 02/12/2022. Further, anti-Dengue IgM antibody-positive samples having high Optical Density (OD) value(s) were selected and subjected to RT PCR to determine the serotype(s) of the Dengue virus.Results:Of the 914 blood samples examined for the presence of Dengue infection, 111 (12.14%) were found positive for anti- Dengue IgM antibody indicating acute infection of Dengue virus.Of the positive patients, there were 56 (50.45%) males and 55 (49.54%) females.Predominant clinical features observed among the Dengue-confirmed patients included – fever (74%), headache (19%), arthralgia / joint pain (9%), myalgia (6%) and vomiting (6%) respectively.The study revealed that while the circulation of three (03) Dengue virus serotypes, namely DENV – 1, DENV – 2 & DENV – 3 were observed in the Tengnoupal district,the circulation of two Dengue virus serotypes i.e., DENV – 1 & DENV – 2 was evident in Imphal West & Bishnupur districts respectively.The present study also reveals the occurrence of Dengue virus serotype – 1 (DENV - 1) in Churachandpur district. Conclusion:The present study reveals the circulation/distribution of three Dengue virus serotypes namely, DENV – 1, DENV – 2 and DENV – 3 among the studied samples.Dengue virusDengue IgM ELISADengue serotype (RT PCR)Dengue Serotypes In Manipur –Findings From A Retrospective Analytical Study In A Tertiary Care HospitalJournal ArticleIndiaLaitonjam Anand Singh, Scientist C Virus Research Diagnostic Laboratory VRDL, Department of Microbiology, Regional Institute of Medical Sciences, Imphal, Manipur , India.Thingujam Suson, Scientist B Virus Research Diagnostic Laboratory VRDL, Department Of Microbiology, Regional Institute Of Medical Sciences, Imphal, Manipur , India.Chongtham Geeta Chanu, Research Assistant Virus Research Diagnostic Laboratory VRDL, Department Of Microbiology, Regional Institute of Medical Sciences, Imphal, Manipur , India.Khuraijam Ranjana Devi, Principal Investigator VRDL and Professor Head, Department Of Microbiology , Regional Institute of Medical Sciences, Imphal, Manipur, India.Heigrujam Rebachandra Singh, CoPrincipal Investigator VRDL Professor, Department Of Microbiology , Regional Institute of Medical Sciences, Imphal, Manipur, India.