Bhattacharyya, ABhattacharya, KBhattacharyya, BRoy, S1995-12-012009-05-271995-12-012009-05-271995-12-01Bhattacharyya A, Bhattacharya K, Bhattacharyya B, Roy S. A study of aggregation of 9-(dicyano-vinyl)julolidine. Indian Journal of Biochemistry & Biophysics. 1995 Dec; 32(6): 442-6http://imsear.searo.who.int/handle/123456789/26368Di(cyano vinyl)julolidine (DCVJ) is a fluorescent probe which has been used to monitor the local mobility of its binding sites on proteins. It shows a concentration dependence of its emission spectrum in water. At higher DCVJ concentrations, a longer wavelength band appears. The latter increases relative to the shorter wavelength band as a function of increased DCVJ concentration. Absorption and excitation spectra indicate that the concentration dependent emission in the longer wavelength is a consequence of association in the ground state and subsequent excimer formation. DCVJ forms two types of complexes with gamma-cyclodextrin, one of which shows the longer wavelength emission band. Analysis of stoichiometry of association also suggests that longer wavelength emission band may be a consequence of association of two molecules of DCVJ in the gamma-cyclodextrin cavity. Possible uses of such excimer formation in biological systems have also been discussed.engFluorescent Dyes --chemistryNitriles --chemistryProteins --chemistryQuinolizines --chemistrySpectrometry, FluorescenceSpectrophotometry, UltravioletJournal Article