Oshiobugie, Momoh JohnsonDamazio, Olanrewaju AnthonyAjetunmobi, Asibiallau OladipupoBabalola, Adenike OmosalewaAdekunle, Oluwasegun MichaelBusari, Nafeesat OlabisiAiyegbeni, Musa Abdullahi2020-10-162020-10-162019-07Oshiobugie Momoh Johnson, Damazio Olanrewaju Anthony, Ajetunmobi Asibiallau Oladipupo, Babalola Adenike Omosalewa, Adekunle Oluwasegun Michael, Busari Nafeesat Olabisi, Aiyegbeni Musa Abdullahi. Phytochemical Analysis and Antiplasmodial (curative) Activities of Methanolic Leaf Extract of Morinda lucida (Ewe Oruwo) in Male Swiss Mice Infected with Plasmodium berghei NK65. International Journal of TROPICAL DISEASE & Health. 2019 Jul; 37(1): 1-132278–1005http://imsear.searo.who.int/handle/123456789/209563Aim:Medicinal plants have been used for the treatment of many infections and diseases including malaria. The study was conducted to determine the effect of in vivoanti-plasmodialand antioxidant properties of the methanolic leaf extract of Morinda lucidain male Swiss albino mice infected with Plasmodium Berghei NK65. Study Design and Methodology:Phytochemical, GC-MS and AAS analyses were determined in the plant. Swiss albino mice were inoculated intraperitoneally with Plasmodium bergheiNK65. Thirty-five (35) mice were grouped into seven groups, five per group. Group A were not infected with P.bergheiNK65.Group B, C and D served as the negative and positive control groups while Group E, F and G mice were treated with 400, 600 and 800 mg/kg body weight of methanolic leaf extract of M. lucida. Haematological parameters were determined in the whole blood using BC-3200 Auto Hematology Analyzer. TP, MDA, CAT, SOD % inhibition, SOD unit and vitamin A were all determined in the liver homogenateusing standard procedures.Results:The GC-MS result of the M. lucidashows the presence of five bioactive compounds. It was also observed that the plant contains the following minerals: iron, magnesium, potassium, phosphorus and copper. Acute toxicity shows that the LD50>000mg/Kg b.wt. The extract caused 30.96%, 32.93% and 67.23% reduction in parasitemia at 400, 600 and 800 mg/kg body weight respectively while chloroquine exerted 96.53% and artesunate exerted 92.03% reduction at 10 mg/kg body weight respectively. The Haematological parameters showed that the plant extractis nothaematotoxic since it significantly (P<0.05) reduced WBC count, and increase RBC, HGB, and HCT values in the treated mice compared to the infected untreated mice. This study shows that the mean lipid peroxidation (MDA) level was significantly decreased in the malaria treated mice (group C, D, E, F and G) compared to the untreated mice (group B). There was also a significant increase in the total protein, catalase, SOD % inhibition, SOD unit and Vitamin A levels in the liver homogenate of animals treated with chloroquine, artesunate and extract of M. lucidacompared to the untreated mice. Conclusions: The study shows that Morinda lucidapossess antiplasmodial activity in male Swiss mice infected with Plasmodium berghei NK 65.Morinda lucidaanti-plasmodial activityAAS, phytochemical analysisbiochemical parameters and Plasmodium berghei NK65 infected Swiss miceJournal ArticleIndiaDepartment of Chemical Sciences,Biochemistry Unit, School of Pure and AppliedSciences, Lagos State Polytechnic, Ikorodu, Lagos, NigeriaDepartment of Chemical Sciences,Chemistry Unit, School of Pure and AppliedSciences, Lagos State Polytechnic, Ikorodu, Lagos, NigeriaDepartment of Chemical Sciences,Chemistry Unit, School of Pure and AppliedSciences, Lagos State Polytechnic, Ikorodu, Lagos, NigeriaDepartment of Chemical Sciences,Biochemistry Unit, School of Pure and AppliedSciences, Lagos State Polytechnic, Ikorodu, Lagos, NigeriaDepartment of Chemical Sciences,Biochemistry Unit, School of Pure and AppliedSciences, Lagos State Polytechnic, Ikorodu, Lagos, NigeriaDepartment of Chemical Sciences,Biochemistry Unit, School of Pure and AppliedSciences, Lagos State Polytechnic, Ikorodu, Lagos, NigeriaHaematology and Blood Transfusion, Lagos University Teaching Hospital LUTH, Idi-Araba, Mushin,Lagos, Nigeria