Sharma, UrvashiMohan, J S S2009-05-282009-05-282006-06-21Sharma U, Mohan JS. Reduction of vitrification in in vitro raised shoots of Chlorophytum borivilianum Sant. & Fernand., a rare potent medicinal herb. Indian Journal of Experimental Biology. 2006 Jun; 44(6): 499-505http://imsear.searo.who.int/handle/123456789/62090Reduction of vitrification in in vitro raised shoots derived from shoot bases and immature floral buds along with inflorescence axis used as explants of C. borivilianum, a rare medicinal herb is described. Shoot multiplication was obtained on MS medium with 2 mg l(-1) benzylaminopurine (BAP) + 0.1 mg l(-1) indole-3-butyric acid (IBA) and MS medium with 2 mg l(-1) kinetin (Kin) + 0.1 mg l(-1) 2,4-dichlorophenoxy acetic acid (2,4-D) from shoot bases and inflorescence axis respectively. Best multiplication rates were obtained from both the explants on MS medium with 2 mg l(-1) BAP. Vitrification of shoots in cultures appeared during the multiplication stage. Culture bottles with aerated caps reduced the vitrification to 80%. Reduction of BAP concentration from 2 mg l(-1) to zero during subsequent subcultures also minimized vitrification. Use of 0.5-2 mg l(-1) Kin produced healthy shoots when compared to BAP. In vitro raised shoots rooted on Knop salts containing iron and vitamins of MS medium, 2 mg l(-1) IBA and 0.1% activated charcoal. About 80% plantlets survived upon soil transfer. Scanning electron microscopic and image analyzer studies reveal the morphological structural differences between the leaves of normal and vitrified plantlets.eng2,4-Dichlorophenoxyacetic Acid --pharmacologyBenzyl Compounds --pharmacologyCytokinins --metabolismHerbal MedicineImage Processing, Computer-AssistedIndoles --pharmacologyKinetin --pharmacologyMicroscopy, Electron, ScanningPlant Roots --metabolismPlant Shoots --metabolismPlants, Medicinal --metabolismPurines --pharmacologyTime FactorsIn Vitro