Hernández-Acosta, EduardoCovarrubias, Antonio De la MoraFernández-Salas, IdelfonsoEscárcega-Ávila, AngélicaOlivera, Isabel PassalacquaJiménez-Vega, Florinda2025-08-132025-08-132025-06Hernández-Acosta Eduardo, Covarrubias Antonio De la Mora, Fernández-Salas Idelfonso, Escárcega-Ávila Angélica, Olivera Isabel Passalacqua, Jiménez-Vega Florinda. Detection of chikungunya virus in Aedes aegypti in Ciudad Juárez, Chihuahua, Mexico. Journal of Vector Borne Diseases . 2025 Jun; 62(2): 226-2320972-9062https://imsear.searo.who.int/handle/123456789/252558Background & objectives: Chikungunya fever is caused by an arbovirus transmitted primarily by Aedes aegypti mosquito, which is prevalent in Mexico along with other pathogens carried by this vector. This study aimed to detect the presence of the chikungunya virus (CHIKV) in Ae. aegypti in a vulnerable area of Ciudad Juárez, Chihuahua. Methods: Mosquitoes were collected from both indoor and outdoor areas of 87 households using BG-GAT and Gravid traps during the fall of 2015. The presence of CHIKV was determined molecularly using reverse transcriptase polymerase chain reaction, and the infection rate was calculated using the PooledInfRate program. Results: A total of 328 female Ae. aegypti mosquitoes were captured, grouped into 131 pools. 66% of them tested positive for CHIKV. The infection rate, as measured by Minimum Infection Rate (MIR), was 26%, and Maximum Likelihood Estimation (MLE) was 15.2%. Interpretation & conclusion: The presence of the infected vector was confirmed in the study area. This first report in the region highlights the need for active epidemiological surveillance to prevent potential chikungunya outbreaks in this border population.Arbovirusesmolecular diagnosisvectorsChikungunyaAedesJournal ArticleIndiaBiotechnology Laboratory Autonomous University of Cd. Juárez (UACJ), Chihuahua, MexicoBiotechnology Laboratory Autonomous University of Cd. Juárez (UACJ), Chihuahua, MexicoRegional Center for Public Health Research, National Institute of Public Health, Chiapas, MexicoBiotechnology Laboratory Autonomous University of Cd. Juárez (UACJ), Chihuahua, MexicoDepartment of Humanities, Institute of Social and Administrative Sciences, Autonomous University of Cd. Juárez (UACJ), Chihuahua, MexicoBiotechnology Laboratory Autonomous University of Cd. Juárez (UACJ), Chihuahua, Mexico