Gupta, R CShukla, O P2015-07-172015-07-171979-06Gupta R C, Shukla O P. Microbial transformation of isonicotinic acid hydrazide and isonicotinic acid by Sarcina sp. Journal of Biosciences. 1979 Jun; 1(2): 223-234.http://imsear.searo.who.int/handle/123456789/159967Metabolism of isonicotinic acid and isoniazid by Sarcina sp. led to the formation of two metabolites which were characterised as 2-hydroxyisonicotinic acid and citrazinic acid. The blue pigment formed during fermentation was shown to be derived from the auto-oxidation of citrazinic acid. 2-Oxo-glutarate accumulated as the major keto acid when isonicotinic acid or isonicotinic acid hydrazide metabolism was inhibited by 1 mM sodium arsenite. Isonicotinic acid, 2-hydroxyisonicotinic acid and 2-oxo-glutarate were oxidised by isonicotinic acid hydrazide or isonicotinic acid-grown cells; citrazinic acid was, however, not oxidised. Isoniazid hydrazine hydrolase, isonicotinic acid and 2-hydroxyisonicotinic acid hydroxylases were detected in the cell-free extract of Sarcina sp. grown on isonicotinic acid hydrazide or isonicotinic acid.enMicrobial transformationisoniazidisonicotinic acidSarcinaArticle