Ahmad, SRoy, P KBasu, S KJohri, B N2009-05-282009-05-281993-04-01Ahmad S, Roy PK, Basu SK, Johri BN. Cholesterol side-chain cleavage by immobilized cells of Rhodococcus equi DSM 89-133. Indian Journal of Experimental Biology. 1993 Apr; 31(4): 319-22http://imsear.searo.who.int/handle/123456789/56643Side-chain cleavage of sterol and extracellular cholesterol oxidase activity were investigated using viable cells of R. equi DSM 89-133 immobilized in polyacrylamide gel. In batch culture, immobilized cells were active in side-chain cleavage of cholesterol for more than 30 days. Free or immobilized cells were incapable of side-chain cleavage in the absence of 2,2' dipyridyl; cholesterol oxidase was, however, produced in both the cases. Maximal activity of the immobilized cells was 60 to 70% of the free cells.eng2,2'-Dipyridyl --pharmacologyCholesterol --metabolismCholesterol Oxidase --biosynthesisDose-Response Relationship, DrugFermentationRhodococcus equi --drug effectsTemperatureJournal Article