Suke, Sanvidhan GPathak, RahulAhmed, Rafat STripathi, A KBanerjee, B D2008-08-162009-05-272008-08-162009-05-272008-08-16Suke SG, Pathak R, Ahmed RS, Tripathi AK, Banerjee BD. Melatonin treatment prevents modulation of cell-mediated immune response induced by propoxur in rats. Indian Journal of Biochemistry & Biophysics. 2008 Aug; 45(4): 278-81http://imsear.searo.who.int/handle/123456789/28025The effect of melatonin, a major secretory product of the pineal gland, in attenuation of propoxur (2-isopropoxy phenyl N-methyl carbamate)-induced modulation of cell-mediated immune (CMI) response was studied in rats. Male Wistar albino rats were exposed to propoxur (a widely used pesticide) orally (10 mg/kg) and/or melatonin (10 mg/kg) orally for 4 weeks. CMI was measured by delayed-type hypersensitivity (DTH), leucocyte and macrophage migration inhibition (LMI and MMI) responses and estimation of cytokines TNF-alpha and IFN-gamma levels. Rats exposed to propoxur for 4 weeks showed significant decrease in DTH, LMI and MMI responses. Propoxur also suppressed TNF-alpha and IFN-gamma production significantly. Administration of melatonin alone caused a significant increase in DTH response. Although there were no changes in the LMI and MMI response, the cytokine levels were significantly increased, as compared to control. Co-administration of melatonin along with propoxur significantly nullified the effect of the pesticide on the CMI response, except DTH and reversed levels of cytokines to near control/normal values. Thus, melatonin treatment considerably attenuated immunomodulation caused by sub-chronic treatment of propoxur in experimental animals.engAdministration, OralAnimalsAntioxidants --administration & dosageCytokines --immunologyHypersensitivity, Delayed --immunologyImmunity, Cellular --drug effectsLeukocytes --drug effectsMacrophages --drug effectsMaleMelatonin --administration & dosagePesticides --antagonists & inhibitorsPineal Gland --chemistryPropoxur --antagonists & inhibitorsRatsRats, WistarTime FactorsTumor Necrosis Factor-alpha --immunologyJournal Article