Rohman, AbdulArifah, Fitriana HayyuIrnawatiAlam, GeminiMuchtaridi, Muchtaridi2020-10-162020-10-162020-04Rohman Abdul, Arifah Fitriana Hayyu, Irnawati, Alam Gemini, Muchtaridi Muchtaridi. The application of FTIR spectroscopy and chemometrics for classification of Mangosteen extract and its correlation with alpha-mangostin. Journal of Applied Pharmaceutical Science. 2020 Apr; 2020 Apr: 149-1542231-3354http://imsear.searo.who.int/handle/123456789/210740Alpha-mangostin is the major component in Mangosteen (Garcinia mangostana Linn) pericarp having severalpharmacological activities including reducing blood pressure, antidiabetic, anticancer, and antioxidants. The objectiveof this study was to develop Fourier transform infrared spectroscopy-multivariate calibration of partial least square(PLS) for quantitative analysis of alpha-mangostin and to classify mangosteen pericarp using principal componentanalysis. Mangosteen pericarps from different locations (Java provinces and South Sulawesi, Republic of Indonesia)were extracted using ethanol and were subjected to high performance liquid chromatography (HPLC) for the analysisof alpha-mangostin and Fourier transform infrared (FTIR) spectroscopy measurements. HPLC was used to determinethe levels of alpha-mangostin and used as actual values during FTIR spectroscopy analysis. The prediction of alphamangostin was obtained from the correlation between actual values and FTIR predicted values and facilitated withthe PLS model. The results showed that the wavenumbers region of 3,825–937 cm−1 offered a reliable model with acoefficient correlation (r) value of 0.9927 and root mean square error of calibration of 0.0831%. The validation modelsalso exhibited the accurate and precise results for the prediction of alpha-mangostin with an r-value of 0.9754 androot mean square error of prediction value of 0.174%. Furthermore, the chemometrics of principal component analysisusing variables of absorbances at selected fingerprint (1,000–800 cm−1) could classify mangosteen pericarp fromdifferent regions. FTIR spectroscopy combined with chemometrics offered a reliable method for quality assurance ofmangosteen pericarpAlpha-mangostinpartial least squareprincipal component analysismangosteen pericarpFTIR spectroscopyJournal ArticleIndiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaFaculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaFaculty of Pharmacy, Universitas Halu Oleo Kendari, Southeast Sulawesi 93232, IndonesiaFaculty of Pharmacy, Hasanuddin University, Makassar, IndonesiaDepartment of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy Universitas Padjadjaran, Sumedang, Indonesia