Prakash, AKasbekar, Durgadas P2002-03-092009-06-012002-03-092009-06-012002-03-09Prakash A, Kasbekar DP. Genes encoding chimeras of Neurospora crassa erg-3 and human TM7SF2 proteins fail to complement Neurospora and yeast sterol C-14 reductase mutants. Journal of Biosciences. 2002 Mar; 27(2): 105-12http://imsear.searo.who.int/handle/123456789/111031The human gene TM7SF2 encodes a polypeptide (SR-1) with high sequence similarity to sterol C-14 reductase, a key sterol biosynthetic enzyme in fungi, plants and mammals. In Neurospora and yeast this enzyme is encoded by the erg-3 and erg24 genes respectively. In an effort to demonstrate sterol C-14 reductase activity for SR-1 we constructed six recombinant genes coding for chimeras of the Neurospora erg-3 and SR-1 protein sequences and tested them for complementation of the Neurospora erg-3 mutant. To our surprise, all the chimeras failed to complement erg-3. A few of the chimeric proteins were also tested against the yeast erg24 mutant, but again there was no complementation. We discuss some reasons that might account for these unexpected findings.engAmino Acid SequenceAnimalsGenetic Complementation TestHumansMembrane Proteins --geneticsMolecular Sequence DataMutagenesis, Site-DirectedNeurospora crassa --enzymologyOxidoreductases --geneticsOxidoreductases Acting on CH-CH Group DonorsRecombinant Fusion Proteins --geneticsSequence AlignmentJournal Article