Bengtson, Nadya GalvãoBengtson, Camilla Regina GalvãoMendes, Fausto MedeirosPinheiro, Sérgio Luiz2012-07-272012-07-272009-07Bengtson Antonio Lucindo, Bengtson Nadya Galvão, Bengtson Camilla Regina Galvão, Mendes Fausto Medeiros, Pinheiro Sérgio Luiz. Histological and radiographic evaluation of the muscle tissue of rats after implantation of bone morphogenic protein (rhBMP-2) in a scaffold of inorganic bone and after stimulation with low-power laser light. Indian Journal of Dental Research. 2010 Jul-Sept; 21(3): 420-424.http://imsear.searo.who.int/handle/123456789/139893Objective: The present study histologically and radiologically evaluates the muscle tissue of rats after implantation of bone morphogenic protein (rhBMP-2) in a natural inorganic bone mineral scaffold from a bull calf femur and irradiation with low-power light laser. Materials and Methods: The right and left hind limbs of 16 rats were shaved and an incision was made in the muscle on the face corresponding to the median portion of the tibia, into which rhBMP-2 in a scaffold of inorganic bone was implanted. Two groups of limbs were formed: control (G1) and laser irradiation (G2). G2 received diode laser light applied in the direction of the implant, at a dose of 8 J/cm2 for three minutes. On the 7th, 21st, 40th and 112th days after implantation, hind limbs of 4 animals were radiographed and their implants removed together with the surrounding tissue for study under the microscope. The histological results were graded as 0=absence, 1=slight presence, 2=representative and 3=very representative, with regard to the following events: formation of osteoid structure, acute inflammation, chronic inflammation, fibrin deposition, neovascularization, foreign-body granuloma and fibrosis. Results: There were no statistically significant differences in these events at each evaluation times, between the two groups (P>0.05; Mann-Whitney test). Nevertheless, it could be concluded that the natural inorganic bone matrix with rhBMP-2, from the femur of a bull calf, is a biocompatible combination. Conclusions: Under these conditions, the inductive capacity of rhBMP-2 for cell differentiation was inhibited. There was a slight acceleration in tissue healing in the group that received irradiation with low-power laser light.enInorganic bone scaffoldrhBMP-2laser lightAbsorbable ImplantsAnimalsBiocompatible Materials --therapeutic useBone Matrix --drug effectsBone Matrix --radiation effectsBone Matrix --transplantationBone Morphogenetic Protein 2Bone Morphogenetic Proteins --administration & dosageBone Morphogenetic Proteins --radiation effectsBone Morphogenetic Proteins --therapeutic useCattleCell Differentiation --drug effectsCell Differentiation --radiation effectsFibrin --analysisFibrosisGranuloma, Foreign-Body --etiologyGranuloma, Foreign-Body --pathologyInflammationLaser Therapy, Low-Level --methodsLasers, Semiconductor --therapeutic useMaleMuscle, Skeletal --pathologyMuscle, Skeletal --radiographyMuscle, Skeletal --surgeryNeovascularization, Physiologic --drug effectsNeovascularization, Physiologic --radiation effectsOsteogenesis --drug effectsOsteogenesis --radiation effectsRadiation DosageRatsRats, WistarRecombinant Proteins --administration & dosageRecombinant Proteins --radiation effectsRecombinant Proteins --therapeutic useTime FactorsTissue ScaffoldsTransforming Growth Factor beta --administration & dosageTransforming Growth Factor beta --radiation effectsTransforming Growth Factor beta --therapeutic useWound Healing --drug effectsWound Healing --radiation effectsArticle