Birru, BhaskarMekala, Naveen KumarParcha, Sreenivasa Rao2020-11-182020-11-182019-03Birru Bhaskar, Mekala Naveen Kumar, Parcha Sreenivasa Rao. Mechanistic role of perfusion culture on bone regeneration. Journal of Biosciences. 2019 Mar; 44(1): 1-90250-59910973-7138http://imsear.searo.who.int/handle/123456789/214295Bone tissue engineering (BTE) aims to develop engineered bone tissue to substitute conventional bone grafts. To achievethis, culturing the cells on the biocompatible three-dimensional (3D) scaffold is one alternative approach. The newfunctional bone tissue regeneration could be feasible by the synergetic combinations of cells, biomaterials and bioreactors.Although the field of biomaterial design/development for BTE applications attained reasonable success, development ofsuitable bioreactor remains still a major challenge. Tissue engineering bioreactors provide the microenvironment requiredfor neo-tissue regeneration, and also can be used to study the physio-chemical cues effect on cell proliferation anddifferentiation in order to produce functional tissue. In this direction, various bioreactors have been developed andevaluated for the successful development of engineered bone tissue. Continues assessment of tissue development andlimitations of the bioreactors lead to the progression of perfusion flow bioreactor system. Improvements in perfusion reactorsystem were able to yield multiple tissue engineered constructs with uniform cell distribution, easy to operate protocols andalso effectively handled for the functional tissue development to meet the adequate supply of engineered graft for clinicalapplication.Bioreactorbone tissue engineeringPhysico-chemical cuesperfusion reactor3D scaffoldsJournal ArticleIndiaDepartment of Biotechnology, National Institute of Technology, Warangal 506 004, TS, IndiaCollege of Medicine, Central Michigan University, Mt Pleasant 48858, USADepartment of Biotechnology, National Institute of Technology, Warangal 506 004, TS, India