Rao, K BTotey, S M2009-05-282009-05-281992-09-01Rao KB, Totey SM. Sex determination in sheep and goats using bovine Y-chromosome specific primers via polymerase chain reaction: potential for embryo sexing. Indian Journal of Experimental Biology. 1992 Sep; 30(9): 775-7http://imsear.searo.who.int/handle/123456789/55750A simple and novel method, using polymerase chain reaction (PCR) has been standardized for accurate sex determination in sheep and goats. The assay utilizes a pair of bovine Y-chromosome specific primers and the genomic DNA isolated from blood samples of adult male and female sheep and goats. The primers recognize and amplify the Y-chromosome specific sequences in male goats and sheep. The assay is accurate, reliable and rapid.engAnimal HusbandryAnimalsBase SequenceBlastocystCattleDNA --geneticsDNA ProbesFemaleGoatsMaleMolecular Sequence DataPolymerase Chain Reaction --methodsPregnancySex Determination (Analysis) --methodsSheepY ChromosomeJournal Article