Kumar, S SampathPuttaraju, H P2013-08-022013-08-022012-12Kumar S Sampath, Puttaraju H P. Improvised microinjection technique for mosquito vectors. Indian Journal of Medical Research. 2012 Dec; 136(6): 971-978.http://imsear.searo.who.int/handle/123456789/147660Background & objectives: Bio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection. Methods: Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult. Results: The improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability. Interpretation & conclusions: The microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability.enBio-manipulationcritical periodmicroinjectionmosquito vectorssurvivabilityArticle