Maurya, Devendra PrasadVats, SiddharthRai, SudheerNegi, Sangeeta2013-11-192013-11-192013-11Maurya Devendra Prasad, Vats Siddharth, Rai Sudheer, Negi Sangeeta. Optimization of enzymatic saccharification of microwave pretreated sugarcane tops through response surface methodology for biofuel. Indian Journal of Experimental Biology. 2013 Nov; 51(11): 992-996.http://imsear.searo.who.int/handle/123456789/149408The optimization of biomass loading enzyme loading, surfactant concentration and incubation time, using response surface methodology (RSM) and Box Behnken design for enzymatic saccharification of sugarcane tops (SCT) for maximum recovery of fermentable sugars using crude cellulases, resulted in 90.24% saccharification efficiency. Maximum saccharification yield of 0.376 g/g glucose as substrate for ethanol production was observed at optimal conditions of 10% biomass loading (pretreated), 100FPU/g of cellulase loading, 0.04% (w/w) surfactant concentration and 72 h of incubation time.enCellulasesMicrowave pre-treatmentSaccharificationSugarcane topBiofuelsBiomassEnzymes --metabolismHydrolysisMicrowavesSaccharum --chemistrySurface PropertiesArticle