Gupta, A KSingh, B KYadav, M P2009-05-282009-05-281996-11-01Gupta AK, Singh BK, Yadav MP. Application of polymerase chain reaction (PCR) for diagnosis of equine herpes virus-1 (EHV-1). Indian Journal of Experimental Biology. 1996 Nov; 34(11): 1077-80http://imsear.searo.who.int/handle/123456789/60863Fifty aborted foetus samples were diagnosed for the presence of equine herpes virus-1 (EHV-1) by polymerase chain reaction (PCR) technique. Specific primer pair for amplification of a particular segment of EHV-1 DNA in gc region having 3 Hae III restriction endonuclease sites was used. A 409 base pair segment obtained as PCR amplification product in 9 samples was digested with Hae III to confirm the presence of EHV-1 as the infectious agent in aborted tissues. It was observed that PCR technique was more sensitive, specific and rapid than the conventional virological diagnostic methods.engAbortion, Veterinary --virologyAnimalsBase SequenceDNA Primers --geneticsFemaleHerpesviridae Infections --diagnosisHerpesvirus 1, Equid --geneticsHorse Diseases --diagnosisHorsesPolymerase Chain Reaction --methodsPregnancyJournal Article