Radhakrishnan, V VMathai, ARadhakrishnan, N SRout, DSehgal, S2009-05-282009-05-281991-07-01Radhakrishnan VV, Mathai A, Radhakrishnan NS, Rout D, Sehgal S. Immunohistochemical demonstration of mycobacterial antigens in intracranial tuberculoma. Indian Journal of Experimental Biology. 1991 Jul; 29(7): 641-4http://imsear.searo.who.int/handle/123456789/62444Mycobacterial antigens have been demonstrated immunohistochemically in the paraffin sections of 10 intracranial tuberculous granulomas and the results were compared with the detection of acid fast bacilli by conventional Ziehl-Neelsen method. In none of the 10 specimens, acid fast bacilli were demonstrated while mycobacterial antigens were characterised as diffusely staining granular brownish-pink material within the cytoplasm of giant cells and macrophages. In 14 specimens of granulomatous lesions due to non-tuberculous aetiology, immunohistochemical stains were negative for mycobacterial antigen. Thus demonstration of mycobacterial antigen will be not only useful in establishing mycobacterial aetiology of a caseating intracranial granuloma but also can be used as an alternative method to the conventional Ziehl-Neelsen method.engAntigens, Bacterial --analysisBrain Diseases --diagnosisGranuloma --immunologyHumansImmunoenzyme TechniquesMycobacterium tuberculosis --immunologyStaining and LabelingTuberculoma --diagnosisComparative Study