Monira, ShirajumShabnam, Syeda AntaraAlam, Nur HaqueEndtz, Hubert PhCravioto, AlejandroAlam, Munirul2016-02-222016-02-222012-09Monira Shirajum, Shabnam Syeda Antara, Alam Nur Haque, Endtz Hubert Ph, Cravioto Alejandro, Alam Munirul. 16S rRNA Gene-targeted TTGE in Determining Diversity of Gut Microbiota during Acute Diarrhoea and Convalescence. Journal of Health, Population and Nutrition. 2012 Sep; 30(3): 250-256.1606-0997http://imsear.searo.who.int/handle/123456789/173784The human gut microbiota play a vital role in health and nutrition but are greatly modified during severe diarrhoea due to purging and pathogenic colonization. To understand the extent of loss during and after diarrhoea, faecal samples collected from children (n=21) suffering from acute diarrhoea and from their healthy siblings (n=9) were analyzed by 16S rRNA gene-targeted universal primer polymerase chain reaction (PCR), followed by temporal temperature gradient gel electrophoresis (TTGE). The gut microbiota decreased significantly as indicated by the number of TTGE bands at day 0 of acute diarrhoea [patients vs healthy siblings: 11±0.9 vs 21.8±1.1 (mean±standard error), p<0.01]. The number of bands showed a steady increase from day 1 to day 7; however, it remained significantly less than that in healthy siblings (15±0.9, p<0.01). These results suggest that appropriate therapeutic and post-diarrhoeal nutritional intervention might be beneficial for the early microbial restoration and recovery.enChildConvalescenceDiarrhoeaDiarrhoeaAcuteGutMicrobiota16S rRNA geneBangladeshArticle