Rajalingam, RMehra, N KSingal, D P2009-05-282009-05-282000-07-24Rajalingam R, Mehra NK, Singal DP. Polymorphism in heat-shock protein 70-1 (HSP70-1) gene promoter region and susceptibility to tuberculoid leprosy and pulmonary tuberculosis in Asian Indians. Indian Journal of Experimental Biology. 2000 Jul; 38(7): 658-62http://imsear.searo.who.int/handle/123456789/59284Heat shock proteins (HSP) act as immunological target structures either by themselves because of an unusual expression pattern, or they are carrier proteins for immunogenic peptides. A three-allele polymorphism of HSP70-1 promoter region was analysed in random patients with pulmonary tuberculosis (PTB), or with tuberculoid (TT) leprosy and healthy controls from North India. HSP70-1A and HSP70-1C occurred more frequently (> 60%) while HSP70-1B occurred infrequently in this population. Only HSP70-1A allele was significantly increased in TT leprosy as compared to healthy controls (91.8% Vs 71.1%, Pc < 0.03, RR = 4.58). Although a strong association of HLA-DR15 was observed with both of these patient groups in earlier studies, no correlation was found between HSP70-1 promoter alleles with any of the HLA allotypes. Amongst six possible genotype combinations of HSP70-1 promoter allele, only four (A/A, A/B, A/C, C/C) were encountered in Asian Indians. A significant increase of HSP70-1 A/C genotype was observed among DR15 negative PTB patients as compared to DR15 negative controls (87.5% Vs 35.7%, X2 = 8.6, Pc < 0.02) giving highest relative risk of 12.6. These findings suggest that HSP70-1 genes may play a secondary role to HLA-DR in governing susceptibility to mycobacterial infectious diseases.engAdultBase SequenceCase-Control StudiesDNA Primers --geneticsFemaleHLA Antigens --geneticsHSP70 Heat-Shock Proteins --geneticsHumansIndiaLeprosy, Tuberculoid --geneticsMalePolymorphism, GeneticPromoter Regions, GeneticTuberculosis, Pulmonary --geneticsJournal Article