Avinash, G.P.Namasivayam, S. Karthick RajaBharani, R.S. ArvindSamrat, K.Tamilselvi, M.2025-05-122025-05-122024-12Avinash G.P., Namasivayam S. Karthick Raja, Bharani R.S. Arvind, Samrat K., Tamilselvi M. . Eco-friendly formulation preparation of fungal biopesticide Metarhizium anisopliae and its impact on the viability, insecticidal activity and pesticidal compatibility. Journal of Environmental Biology. 2024 Dec; 45(6): 686-6940254-87042394-0379https://imsear.searo.who.int/handle/123456789/247543Aim: The present investigation evaluated the effect of various formulations on the viability, pesticide compatibility and biocontrol potential of fungal biopesticidal agent Metarhizium anisopliae under laboratory conditions. Methodology: Soil isolate of M. anisopliae biomass was formulated with different oils like sunflower, groundnut, neem oil, water and diesel using green principles. Results: Sunflower oil recorded maximum viability among the formulations, revealing 91.4% formulation efficacy, followed by groundnut oil, which showed 87.2% efficacy. The viability of the fungal isolate was found to be low in diesel formulation, which showed 69.2% formulation efficacy. A compatibility study revealed that sunflower oil formulation showed high tolerance to all the tested pesticides. Sunflower oil formulation also showed a high rate of mortality against S. litura. Interpretation: The present study suggests using oil formulation as an effective carrier for the fungal biopesticide Metarhizium anisopliae with high compatibility and viability.BiopesticideFungal strainsMetarhizium anisopliaeSunflower oilEco-friendly formulation preparation of fungal biopesticide Metarhizium anisopliae and its impact on the viability, insecticidal activity and pesticidal compatibilityJournal ArticleIndiaCentre for Applied Research, SaveethaSchool of Engineering, Saveetha Institute of Medical and Technical Sciences, Chennai?602 105, IndiaCentre for Applied Research, SaveethaSchool of Engineering, Saveetha Institute of Medical and Technical Sciences, Chennai?602 105, IndiaInstitute of Obstetrics and Gynaecology, Madras Medical College, Chennai?600 008, IndiaDepartment of Biotechnology, M.S. Ramaiah Institute of Technology, Bengaluru?560 054, IndiaCentre for Bio-resource Research and Development (C-BIRD), Sathyabama Institute of Science and Technology, Chennai ? 600119, India